what is cccp used for in bacteria researchgate

In fact, they probably are in about 75 percent of people who ultimately have ' rheumatoid arthritis. These types of antibodies are not present in everybody with rheumatoid arthritis. In rheumatoid arthritis, patients with rheumatoid arthritis begin to make antibodies against these particular proteins, and it turns out to be a very specific laboratory finding in rheumatoid arthritis. And it's a particular type of protein that some patients or some people will have in their bodies. Growth medium pH, probably, determines the character of interaction of the TrkA with the F0F1.— - Question: What is an anti-CCP test and how is it used to diagnose rheumatoid arthritis?Īnswer: CCP stands for cyclic citrullinated peptide. K+ uptake in the bacteria grown anaerobically at alkaline pH is carried out by the TrkA system, which functions as uniporter, interacts with the F0F1 proton pump by means of transmembrane electrochemical gradient for H+ which is used as the driving force. We suggest that proton and potassium transport systems are involved in the regulation of intracellular pH in E. The DCCD-inhibited ATPase activity of membrane vesicles did not show any significant dependence on K+ activity in the medium. coli mutants with the defect in the alpha-subunit of H(+)-ATPase F0F1 complex (uncA) or in the TrkA system of K+ uptake (trkA trkD). This H+,K+ exchange was not observed in E. The stoichiometry of DCCD-inhibited cation fluxes was unstable upon change of experimental conditions. H+ secretion was sensitive to osmotic shock, and K+ uptake up to the potassium gradient between the cytoplasm and the medium of more than 2 x 10(3) occurred at Km 3.0 mM and was carried out upon upshock or downshock. Proton-potassium exchange proceeded in one step, was inhibited by the N,N'-dicyclohexylcarbodiimide (DCCD) and protonophore CCCP. Fermenting Escherichia coli wild type cells, grown anaerobically at alkaline pH (pH 8.3-8.6), upon transfer into the medium at pH 7.5-7.8 were shown to maintain intracellular pH at 7.5, acidify medium, take in K+, generate membrane potential of -160 mV and produce molecular hydrogen.

What is cccp used for in bacteria researchgate